Wednesday, November 20, 2002

So little time: Jos� Bov� sentenced to 14 months [NYT]; Le Monde weighs in (rather gaulishly)
Pourtant, il est clair que Jos� Bov� n'est pas un d�linquant comme les autres. Il agit en militant politique, au service d'une cause, qu'il d�fend � sa mani�re, et il serait normal qu'il soit jug� comme tel.
You mean, by sending him to jail for breaking the law? [cf. this bilingual reactionary].
Forbes has a dilemma:
Increasing numbers of large corporations find themselves caught between two seemingly contradictory goals: satisfying investors' expectations for progressive earnings growth and consumers' growing demand for social responsibility.
And a very interesting paper [requires subscription to Blackwell-Synergy] in the new Plant Journal. I will attempt to decipher it later -- here's the abstract:
To more fully characterize the internal structure of transgene loci and to gain further understanding of mechanisms of transgene locus formation, we sequenced more than 160 kb of complex transgene loci in two unrelated transgenic oat (Avena sativa L.) lines transformed using microprojectile bombardment. The transgene locus sequences from both lines exhibited extreme scrambling of non-contiguous transgene and genomic fragments recombined via illegitimate recombination. A perfect direct repeat of the delivered DNA, and inverted and imperfect direct repeats were detected in the same transgene locus indicating that homologous recombination and synthesis-dependent mechanism(s), respectively, were also involved in transgene locus rearrangement. The most unexpected result was the small size of the fragments of delivered and genomic DNA incorporated into the transgene loci via illegitimate recombination; 50 of the 82 delivered DNA fragments were shorter than 200 bp. Eleven transgene and genomic fragments were shorter than the DNA lengths required for Ku-mediated non-homologous end joining. Detection of these small fragments provided evidence that illegitimate recombination was most likely mediated by a synthesis-dependent strand-annealing mechanism that resulted in transgene scrambling. Taken together, these results indicate that transgene locus formation involves the concerted action of several DNA break-repair mechanisms.


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